解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::HIV-1 replicative activity and its relation to the clinical and immunological evolution of infection was studied in a group of 150 HIV-1 seropositive Italian i.v. drug abusers over a 1 year period. HIV-1 was isolated from 90 (60%) subjects; two groups of isolates were distinguished, according to replicative activity "...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890320411
更新日期:1990-12-01 00:00:00
abstract::Nine ducks congenitally infected with the duck hepatitis B virus (DHBV) were treated either orally (four ducks for 10 weeks) or intraperitoneally (five ducks for 12 weeks) with the Indian traditional herbal remedy Phyllanthus amarus. Compared to placebo-treated control ducks, these treatments did not result in a reduc...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890320404
更新日期:1990-12-01 00:00:00
abstract::The TD97 strain vaccine virus was prepared from the Tanabe strain measles virus by low-temperature passages in primary cell cultures and ultraviolet (UV) mutagenesis. The TD97 strain exhibited the following characteristics: highly temperature sensitive, neither multiplying nor forming any plaques at 40 degrees C in Ve...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890320311
更新日期:1990-11-01 00:00:00
abstract::The immune response to guinea pig cytomegalovirus (gpCMV) was evaluated by immunoblotting. Preinoculation guinea pig plasma did not react with gpCMV antigen, whereas convalescent plasma reacted to at least 18 gpCMV-specific polypeptides. The initial immune response was primarily directed at polypeptides with MWs of 10...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890320305
更新日期:1990-11-01 00:00:00
abstract::Saliva and peripheral blood samples from 20 healthy adults were examined for the presence of human herpesvirus-6 (HHV-6) DNA sequences using the polymerase chain reaction. Eighteen out of 20 whole saliva samples contained detectable HHV-6 genomes. The majority of peripheral blood samples were also positive; however, t...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890320113
更新日期:1990-09-01 00:00:00
abstract::Clinical, virological, and histological findings in four HBsAg chronic carriers who cleared HBV markers from both serum and liver following HDV superinfection are described. The patients were long-term HBsAg carriers and all were HBV-DNA/HBeAg positive. Liver biopsy, obtained from three of the patients between 5 and 1...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310408
更新日期:1990-08-01 00:00:00
abstract::HIV excretion patterns and specific antibody responses were evaluated in blood, semen, female genital secretions, saliva, and crevicular fluid. Samples were examined for infectious virus, viral antigens, viral nucleic acid, HIV specific IgG, IgA, anti-nef, and anti-p24. Viral load in peripheral blood appeared to incre...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310409
更新日期:1990-08-01 00:00:00
abstract::To assess the prevalence of HPV infection in the genital tracts of women with normal PAP smears, a random series of 109 women was reexamined using colposcopy, a further PAP smear, and punch biopsies taken from the cervix (in 33 cases), vagina (212 cases), and anus (20 cases). The biopsy material was examined using rou...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310404
更新日期:1990-08-01 00:00:00
abstract::In earlier studies, hepatitis E virus (HEV) particles were detected in the stools of patients with enterically transmitted non-A, non-B (ENANB) hepatitis, and HEV was etiologically associated with this disease. Such particles have not been observed in the liver, however. We describe the pathological findings in the li...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310310
更新日期:1990-07-01 00:00:00
abstract::A cDNA copy of the HCV229E nucleocapsid protein gene was isolated and characterized. Sequence analysis predicts a nucleocapsid polypeptide of 389 amino acids with a molecular weight (mol. wt.) of 43,450. Single strand RNA probes derived from the cDNA copy hybridize specifically to HCV229E RNA and approximately 50 pg o...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890310216
更新日期:1990-06-01 00:00:00
abstract::Paired serum and saliva samples were tested by enzyme-linked immunosorbent assay (ELISA) and Western blot (WB) for the presence of human immunodeficiency virus (HIV) antibodies. The study group included 36 individuals known to be HIV seropositive and 14 healthy, seronegative controls. HIV antibodies were detected in a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890300403
更新日期:1990-04-01 00:00:00
abstract::A sensitive and specific nonradioactive in situ hybridization method capable of detecting single-copy integrated hepatitis B virus (HBV) DNA sequences in hepatocellular carcinoma (HCC) cell lines was developed. In situ hybridization of biotinylated HBV (adr, adw) DNA probes with nine different human HCC cell lines wer...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890300407
更新日期:1990-04-01 00:00:00
abstract::Recently a conventional method for the laboratory diagnosis of human cytomegalovirus (HCMV) infection was improved by using centrifugation culture to enhance viral adsorption and by detecting HCMV early antigen and DNA. Comparison of the sensitivity of three rapid methods using commercial diagnostic reagents for the d...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890300204
更新日期:1990-02-01 00:00:00
abstract::Coronavirus-like particle(s) (CVLP) are faecal-derived pleomorphic membrane bound virus-like particles characterised by a fringe of club-shaped spikes that measure about 27 nm in length. The association of CVLP with a variety of social, clinical, and epidemiological factors was examined after a 69 month survey of faec...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890290404
更新日期:1989-12-01 00:00:00
abstract::The nature of the immunodominant a region of hepatitis B virus surface antigen (HBsAg) has been examined by mutation of specific amino acid residues. Proline 142 was required for the exhibition of full antigenicity. Replacement of cysteines 124 and 147 by serines drastically reduced or eliminated reactivity with antib...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890290310
更新日期:1989-11-01 00:00:00
abstract::Antibodies to herpes simplex virus and Epstein-Barr virus were sought in the sera of 33 patients with endoscopically proved duodenal ulcer and 33 healthy controls. Neither the prevalence nor the antibody levels to both viruses were significantly different between the two groups. These results suggest that herpes virus...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890290314
更新日期:1989-11-01 00:00:00
abstract::Patients with acquired immunodeficiency syndrome (AIDS) commonly suffer from opportunistic infections associated with members of the herpes virus family. To investigate whether certain of these other viruses might have an effect on the ability of the human immunodeficiency virus type 1 (HIV-1) to replicate, we coincub...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890290207
更新日期:1989-10-01 00:00:00
abstract::Foscarnet (trisodium phosphonoformate) is a novel antiviral agent that inhibits viral-specific DNA polymerase. In the present study, eight males with chronic HBV carriage (HBeAg and HBV-DNA seropositivity greater than 12 months) showing chronic persistent hepatitis (CPH) or chronic active hepatitis (CAH) on liver biop...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890290214
更新日期:1989-10-01 00:00:00
abstract::From the Pacific Republic of Kiribati, 90/130 (69%) of hepatitis B-infected individuals carry antibodies to hepatitis delta virus. The prevalence of delta infection varied between geographically isolated subgroups in the population and delta antibodies were found more frequently in subjects over the age of 10. It is u...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890290210
更新日期:1989-10-01 00:00:00
abstract::With virus-infected autologous and allogenic mononuclear cells as specific targets, the development of cell-mediated cytotoxic reactivity to respiratory syncytial virus (RSV) was studied in peripheral blood lymphocytes (PBL) in groups of infants with acute RSV infection and in other control groups of subjects during a...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890280304
更新日期:1989-07-01 00:00:00
abstract::A total of 41 stool rotavirus specimens collected from children with acute diarrhea at four different locations in Akita Prefecture, Japan, during the peak of the winter diarrhea epidemic in 1988 were analyzed by polyacrylamide gel electrophoresis of viral RNA in conjunction with subgrouping assay. We found that a sin...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890280311
更新日期:1989-07-01 00:00:00
abstract::Shedding of herpes simplex virus type 1 (HSV-1) into saliva was studied in 83 patients with orofacial fractures. Infectious virus was isolated from 14 of 83 patients (16.8%) with no detectable herpetic lesion during hospitalization. Of the 83 patients, 44 (53%) had HSV-1 specific antibody. Virus shedding into saliva w...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890280205
更新日期:1989-06-01 00:00:00
abstract::Monoclonal antibodies were used for detecting varicella-zoster virus (VZV) by immunofluorescence (IF) in clinical specimens inoculated on shell vial cultures. Vesicles of 74 patients with varicella or herpes zoster-like eruptions were tested by this method and by conventional cell culture. Further diagnostic tests wer...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890280102
更新日期:1989-05-01 00:00:00
abstract::A new assay was developed for the detection of hepatitis B virus (HBV) in human serum using amplification of a short viral DNA sequence by means of the polymerase chain reaction. As little as 0.4 fg viral DNA, corresponding to about 130 genome equivalents, per ml serum could be detected after the amplification procedu...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890270409
更新日期:1989-04-01 00:00:00
abstract::Previous studies demonstrated that in vitro infection with influenza A viruses altered several functions of human monocytes-macrophages but did not detectably alter functions of human lymphocytes. However, both types of cells were infected, as determined by production and surface expression of viral antigens. In the c...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890270302
更新日期:1989-03-01 00:00:00
abstract::To establish gut immunity in monkeys to polio-virus type 1, we fed four juvenile bonnet monkeys (Macaca radiata) with 100 median infectious doses of the Mahoney strain of virus. The duration of viral shedding in the throat and faeces and the titre of virus in faeces were measured. Eighteen days after the last monkey s...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890270208
更新日期:1989-02-01 00:00:00
abstract::Anti-influenza serum and nasal antibody titers were followed during a double-blind, placebo-controlled, randomized study assessing the prophylactic efficacy of 50, 100, and 200 mg/day of amantadine hydrochloride against experimental challenge with influenza A/Beth/1/85. The geometric mean titers (GMT) of serum hemaggl...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1002/jmv.1890270213
更新日期:1989-02-01 00:00:00
abstract::The specificity and neutralizing activity of antibodies against the major herpes simplex virus type 1 (HSV-1) glycoproteins were tested in serum samples of patients with a history of HSV-1 infection. By preabsorption of sera to preparations of native and denatured HSV-1 proteins, followed by immunoblotting and microne...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890270113
更新日期:1989-01-01 00:00:00
abstract::Live attenuated cold-adapted influenza vaccine is undergoing evaluation in man. Several strains have proven to be safe, immunogenic, nontransmissible, and protective against experimental challenge. In this study of A/Peking/2/79(H3N2), with six internal genes from the cold-adapted (Ca) parent A/Ann Arbor/6/60(H2N2), w...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890260209
更新日期:1988-10-01 00:00:00
abstract::Sera from 31 human immunodeficiency virus (HIV)-infected patients, representing different clinical stages of HIV infection, were assayed for antibodies against measles and mumps viruses by various serological tests and compared to 23 healthy controls. Sera from four patients (two primary, one asymptomatic, and one acq...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890260107
更新日期:1988-09-01 00:00:00
abstract::A diabetogenic strain of coxsackievirus B4 of human origin has been purified to study its biochemistry and diabetogenicity. Tissue culture cells infected with the virus contain two distinct types of particles--virions and membrane-bound virions (MBV). MBVs are lighter (p = 1.29) than virions (p = 1.34), and they conta...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890260109
更新日期:1988-09-01 00:00:00
abstract::Sera of patients from China with hepatocellular carcinoma (HCC) were tested for the presence of HBc/HBe- and HBx antibodies by immunoblotting using recombinant MS2 or beta gal fusion proteins as substrate. Antibodies against HBx were detected in four out of 68 HBsAg positive and in one out of three HBsAg negative sera...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890250310
更新日期:1988-07-01 00:00:00
abstract::A viral surveillance system in Nashville detected an outbreak of influenza B that occurred between January and March 1986. Paired sera from 32 individuals with culture-documented influenza B illness were tested using three serologic assays. Enzyme-linked immunosorbent assay (ELISA) using purified hemagglutinin-neurami...
journal_title:Journal of medical virology
pub_type: 临床试验,杂志文章
doi:10.1002/jmv.1890250313
更新日期:1988-07-01 00:00:00
abstract::The live varicella vaccine has been recommended for use in immunocompromised subjects and in adults who are susceptible to chickenpox. However, we do not know how humoral and cell-mediated immune responses induced after vaccination differed from those obtained after natural infection. To answer this, 45 previously inf...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890250207
更新日期:1988-06-01 00:00:00
abstract::A cohort of 127 nursing home residents aged 60-98 years were vaccinated during the winter of 1985-86 with the A-Chile 1/83 (C), A-Philippines 2/82 (P), and B-USSR (B) commercial influenza vaccines. Before vaccination 40%, 23%, and 69% were susceptible to influenza Ac, Ap, and B, respectively [hemagglutinin inhibition ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890250112
更新日期:1988-05-01 00:00:00
abstract::The replication strategy of a human serotype 1 rotavirus, adapted to rapid growth in CV-1 cells, was investigated. A single cycle growth curve revealed eclipse and latent periods of 3 and 4 hours, respectively. Although the extent of reduction of host cell protein synthesis was directly related to the multiplicity of ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890250113
更新日期:1988-05-01 00:00:00
abstract::Cytomegalovirus-specific complement-fixing IgM (CMV CF-IgM) antibody was detected in 12/84 (14.3 percent) sera containing CMV-specific IgM. Higher titres were obtained by using a purified antigen preparation containing enveloped virus particles and membrane fragments when compared with a crude extract of infected cell...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890240312
更新日期:1988-03-01 00:00:00
abstract::Rabbit antibodies produced by immunization with complete hepatitis A virions (HAV) recognized all the viral structural proteins and neutralized HAV infectivity in cell culture. Rabbit antibodies to chromatographically purified individual viral proteins and to synthetic peptides representing epitopes on the structural ...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890240212
更新日期:1988-02-01 00:00:00
abstract::The prevalence rate of IgG antibodies to cytomegalovirus (CMV) was determined in a sample of 567 women of childbearing age in the southern part of Israel by the immunoperoxidase assay to membrane antigen (IPAMA) technique. Urban Jewish women of Afro-Asian origin showed significantly higher rates of seropositivity than...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890240104
更新日期:1988-01-01 00:00:00
abstract::The incidence and the outcome of cytomegalovirus (CMV) infections were evaluated in 83 adult recipients of allogenic bone marrow transplantation. Virological and serological surveillance was performed weekly for 3 months posttransplant, and then every other week or every month until 1 year. CMV infection occurred in 4...
journal_title:Journal of medical virology
pub_type: 杂志文章
doi:10.1002/jmv.1890230412
更新日期:1987-12-01 00:00:00